| Citation key |
ISI:000433403900003 |
| Author |
Gutierrez-Sanz, Oscar and Forbig, Enrico and Batista, Ana P. and Pereira, Manuela M. and Salewski, Johannes and Mroginski, Maria A. and Goetz, Robert and De Lacey, Antonio L. and Kozuch, Jacek and Zebger, Ingo |
| Pages |
5703-5711 |
| Year |
2018 |
| ISSN |
0743-7463 |
| DOI |
10.1021/acs.langmuir.7b04057 |
| Address |
1155 16TH ST, NW, WASHINGTON, DC 20036 USA |
| Journal |
LANGMUIR |
| Volume |
34 |
| Number |
20 |
| Month |
MAY 22 |
| Publisher |
AMER CHEMICAL SOC |
| Abstract |
Respiratory complex I (CpI) is a key player in the way organisms obtain energy, being an energy transducer, which couples nicotinamide adenine dinucleotide (NADH)/quinone oxidoreduction with proton translocation by a mechanism that remains elusive so far. In this work, we monitored the function of CpI in a biomimetic, supported lipid membrane system assembled on a 4-aminothiophenol (4-ATP) self-assembled monolayer by surface-enhanced infrared absorption spectroscopy. 4-ATP serves not only as a linker molecule to a nanostructured gold surface but also as pH sensor, as indicated by concomitant density functional theory calculations. In this way, we were able to monitor NADH/quinone oxidoreduction-induced transmembrane proton translocation via the protonation state of 4-ATP, depending on the net onentation of CpI molecules induced by two complementary approaches. An associated change of the amide I/amide II band intensity ratio indicates conformational modifications upon catalysis which may involve movements of transmembrane helices or other secondary structural elements, as suggested in the literature. |
| Bibtex Type of Publication |
Article |
